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August 18, 2019
DOI:
1Laboratory of Molecular Biology, Department of Plant Sciences,Wageningen University & Research, 2Center of Technology for Agricultural Production,Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing University of Agriculture
Summary
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Parasponia andersonii è un albero tropicale in rapida crescita che appartiene alla famiglia Cannabis (Cannabaceae) e può formare noduli di radice che fissano l'azoto in associazione con il rizobium. Qui, descriviamo un protocollo dettagliato per le analisi genetiche inverse in P. andersonii basato su Agrobacterium tumefaciens- mediato trasformazione stabile e modifica del genoma CRISPR/Cas9.
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Cite this Article
Wardhani, T. A., Roswanjaya, Y. P., Dupin, S., Li, H., Linders, S., Hartog, M., Geurts, R., van Zeijl, A. Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii. J. Vis. Exp. (150), e59971, doi:10.3791/59971 (2019).
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