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Transforming, GenomEditing und Phenotypisierung der Stickstoff-fixierenden tropischen Cannabaceae Baum Parasponia andersonii
JoVE Journal
Genetics
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JoVE Journal Genetics
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
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Transforming, GenomEditing und Phenotypisierung der Stickstoff-fixierenden tropischen Cannabaceae Baum Parasponia andersonii

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12,381 Views

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12:22 min

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August 18, 2019

DOI:

10.3791/59971-v

DOI:
10.3791/59971-v

•

12:22 min
August 18, 2019

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12 Views
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1Laboratory of Molecular Biology, Department of Plant Sciences,Wageningen University & Research, 2Center of Technology for Agricultural Production,Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing University of Agriculture

Summary

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Parasponia andersonii ist ein schnell wachsender tropischer Baum, der zur Cannabis-Familie (Cannabaceae) gehört und in Verbindung mit dem Rhizobium stickstofffixende Wurzelknollen bilden kann. Hier beschreiben wir ein detailliertes Protokoll für reverse genetische Analysen in P. andersonii basierend auf Agrobacterium tumefaciens-vermittelte stabile Transformation und CRISPR/Cas9-basierte Genombearbeitung.

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Parasponia AndersoniiNitrogen-fixingTropical TreeCannabaceaeGenome EditingPhenotypingTransgenic Mutant LinesRhizobial SymbiosisIn Vitro PropagationSeed GerminationPlant Transformation

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Wardhani, T. A., Roswanjaya, Y. P., Dupin, S., Li, H., Linders, S., Hartog, M., Geurts, R., van Zeijl, A. Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii. J. Vis. Exp. (150), e59971, doi:10.3791/59971 (2019).

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