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09:21 min
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September 13, 2019
DOI:
1Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego
Summary
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Этот протокол описывает воспроизводимый метод для изоляции мышей рабдомиосаркомы первичных клеток, формирования опухолевой сферы и лечения, и аллотрансплантата трансплантации, начиная с опухолевых культур.
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Cite this Article
Boscolo Sesillo, F., Sacco, A. Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas. J. Vis. Exp. (151), e59897, doi:10.3791/59897 (2019).
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