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Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
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09:21 min
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September 13, 2019
DOI:
1Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego
Summary
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This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.
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Boscolo Sesillo, F., Sacco, A. Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas. J. Vis. Exp. (151), e59897, doi:10.3791/59897 (2019).
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