需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
7,015 Views
•
09:21 min
•
September 13, 2019
DOI:
1Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego
Summary
Automatically generated
This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.
Read Article
Cite this Article
Boscolo Sesillo, F., Sacco, A. Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas. J. Vis. Exp. (151), e59897, doi:10.3791/59897 (2019).
Copy