Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Tumorsphere Derivation and Treatment from Primary …
JoVE Journal
Genetics

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Genetics
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
 

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

Article DOI: 10.3791/59897-v • 09:21 min • September 13th, 2019
September 13th, 2019 •
1,2,3, 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

Chapitres

résumé
Automatic Translation

This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.

Tags

Tumorsphere Derivation Treatment Primary Tumor Cells Mouse Rhabdomyosarcomas Soft-tissue Sarcomas Histologically Myogenic Markers Cell Of Origin Reproducible Methods Reliable Methods Freshly Harvested Tumor Tissues Tumorsphere Formation Assay Advantages Cellular Properties Expansion Enrichment Specific Cell Population Spheroid Structure Tumor Environment Drug-screening Studies Optimization Of Culture Conditions
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter