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09:21 min
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September 13, 2019
DOI:
1Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego
Summary
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Ce protocole décrit une méthode reproductible pour l'isolement des cellules primaires de rhabdomyosarcoma de souris, de la formation et du traitement de la tumorsphere, et de la transplantation d'allogreffe commençant des cultures de tumorspheres.
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Boscolo Sesillo, F., Sacco, A. Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas. J. Vis. Exp. (151), e59897, doi:10.3791/59897 (2019).
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