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Tuning Degradation to Achieve Specific and Efficient Protein Depletion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

DOI:
10.3791/59874-v

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05:11 min

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July 20, 2019

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, , , ,
1Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh, 2Department of Plant Sciences,University of Cambridge, 3Sir William Dunn School of Pathology,University of Oxford

Chapters

  • 00:04Title
  • 00:36General Procedure for Depletion
  • 03:31Results: Analysis of the Tuned Depletion Rate
  • 04:25Conclusion

Summary

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Here, we present a protocol to effectively and specifically deplete a protein of interest in the yeast Saccharomyces cerevisiae using the β-est AID system.

Tags

Keywords: Protein DepletionEssential ProteinProtein FunctionProtein RemovalAuxinBeta-estradiolIAACell DeathCell CultureOptical DensitySample CollectionCentrifugation

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