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Immunology and Infection
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Ajustando a degradação para conseguir a depleção de proteína específica e eficiente
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Ajustando a degradação para conseguir a depleção de proteína específica e eficiente

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5,911 Views

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05:11 min

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July 20, 2019

DOI:

10.3791/59874-v

DOI:
10.3791/59874-v

•

05:11 min
July 20, 2019

•

4 Views
, , , ,
1Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh, 2Department of Plant Sciences,University of Cambridge, 3Sir William Dunn School of Pathology,University of Oxford

Summary

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Aqui, apresentamos um protocolo para efetivamente e especificamente esgotar uma proteína de interesse na levedura Saccharomyces cerevisiae usando o sistema β-est Aid.

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Protein DepletionEssential ProteinProtein FunctionProtein RemovalAuxinBeta-estradiolIAACell DeathCell CultureOptical DensitySample CollectionCentrifugation

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Cite this Article

Barrass, J. D., Mendoza-Ochoa, G. I., Maudlin, I. E., Sani, E., Beggs, J. D. Tuning Degradation to Achieve Specific and Efficient Protein Depletion. J. Vis. Exp. (149), e59874, doi:10.3791/59874 (2019).

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