/

Immunology and Infection

/

特定および効率的なタンパク質枯渇を達成するための分解性の調整

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Please note that all translations are automatically generated. Click here for the English version.

特定および効率的なタンパク質枯渇を達成するための分解性の調整

5,911 Views

•

05:11 min

•

July 20, 2019

DOI:

10.3791/59874-v

DOI:

10.3791/59874-v

•

05:11 min

July 20, 2019

•

4 Views

J. David Barrass¹, Gonzalo I. Mendoza-Ochoa^1,2, Isabella E. Maudlin^1,3, Emanuela Sani¹, Jean D. Beggs¹

¹Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh, ²Department of Plant Sciences,University of Cambridge, ³Sir William Dunn School of Pathology,University of Oxford

Summary

ここで、β-est AIDシステムを用いて酵母サッカロマイセスセレビシエに関心のあるタンパク質を効果的かつ具体的に枯渇させるプロトコルを提示する。

Explore More Videos

Protein Depletion Essential Protein Protein Function Protein Removal Auxin Beta-estradiol IAA Cell Death Cell Culture Optical Density Sample Collection Centrifugation

Read Article