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Developmental Biology
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通过乙基甲烷硫素突变在小谷物作物中靶向诱导局部病变(TILLING)种群的发展
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Developmental Biology
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JoVE Journal
Developmental Biology
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
Please note that all translations are automatically generated.
Click here for the English version.
通过乙基甲烷硫素突变在小谷物作物中靶向诱导局部病变(TILLING)种群的发展
DOI:
10.3791/59743-v
•
08:36 min
•
July 16, 2019
•
Lovepreet Singh
,
Adam Schoen
,
Alexander Mahlandt
,
Bhavit Chhabra
,
James Steadham
,
Vijay Tiwari
,
Nidhi Rawat
1
Department of Plant Science and Landscape Architecture
,
University of Maryland, College Park
Chapters
00:04
Title
00:43
Preparation of Dosage Curve for Effective Mutagenesis
03:09
Cel-1 Assay for Genetic Characterization of Mutants
06:03
Results: EMS Mutation
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述是使用乙基甲酸酯 (EMS) 作为诱变剂在小谷物作物中开发靶向诱导局部病变(TILLING)人群的协议。还提供了使用Cel-1测定突变检测的协议。
Tags
TILLING
Mutagenesis
EMS
Small Grain Crops
Functional Genomics
Gene Discovery
Cel-1 Assay
Mutation Detection
Dosage Optimization
M2 Plants
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