/
/
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
11,218 Views
•
08:36 min
•
July 16, 2019
DOI:
Summary
Automatically generated
Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.
Read Article
Cite this Article
Singh, L., Schoen, A., Mahlandt, A., Chhabra, B., Steadham, J., Tiwari, V., Rawat, N. Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis. J. Vis. Exp. (149), e59743, doi:10.3791/59743 (2019).
Copy