/
/
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
11,167 Views
•
08:36 min
•
July 16, 2019
DOI:
Summary
Automatically generated
Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.
Read Article
Cite this Article
Singh, L., Schoen, A., Mahlandt, A., Chhabra, B., Steadham, J., Tiwari, V., Rawat, N. Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis. J. Vis. Exp. (149), e59743, doi:10.3791/59743 (2019).
Copy