Journal
/
/
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

11,218 Views

08:36 min

July 16, 2019

DOI:

08:36 min
July 16, 2019

8 Views
, , , , , ,

Summary

Automatically generated

Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.

Read Article