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Generation von Organellen von Maus extrahepatische Gallenwege
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Organoids from Mouse Extrahepatic Bile Ducts
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Generation von Organellen von Maus extrahepatische Gallenwege

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9,426 Views

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09:13 min

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April 23, 2019

DOI:

10.3791/59544-v

DOI:
10.3791/59544-v

•

09:13 min
April 23, 2019

•

7 Views
, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Summary

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Dieses Protokoll beschreibt die Herstellung einer Maus extrahepatische Gallenwege 3-dimensionale organoide Systems. Diese biliären Organellen können in Kultur Cholangiocyte Biologie gepflegt werden. Biliären Organellen express Marker der Stammvater und biliären Zellen und polarisierten epithelialen Zellen zusammengesetzt sind.

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Cite this Article

Shiota, J., Zaki, N. H. M., Merchant, J. L., Samuelson, L. C., Razumilava, N. Generation of Organoids from Mouse Extrahepatic Bile Ducts. J. Vis. Exp. (146), e59544, doi:10.3791/59544 (2019).

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