Journal
/
Developmental Biology
/
Generation von Organellen von Maus extrahepatische Gallenwege
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Generation of Organoids from Mouse Extrahepatic Bile Ducts
Please note that all translations are automatically generated. Click here for the English version.

Generation von Organellen von Maus extrahepatische Gallenwege

Deutsch

Automatically Generated

9,395 Views

•

09:13 min

•

April 23, 2019

DOI:

10.3791/59544-v

DOI:
10.3791/59544-v

•

09:13 min
April 23, 2019

•

7 Views
, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Summary

Automatically generated

Dieses Protokoll beschreibt die Herstellung einer Maus extrahepatische Gallenwege 3-dimensionale organoide Systems. Diese biliären Organellen können in Kultur Cholangiocyte Biologie gepflegt werden. Biliären Organellen express Marker der Stammvater und biliären Zellen und polarisierten epithelialen Zellen zusammengesetzt sind.

Explore More Videos

OrganoidsExtrahepatic Bile DuctsMurineCholangiopathiesPreclinical ModelsCholangiocyteCell-to-cell InteractionsBile Duct IsolationDissociationBasement MatrixCell Culture

Read Article

Cite this Article

Shiota, J., Zaki, N. H. M., Merchant, J. L., Samuelson, L. C., Razumilava, N. Generation of Organoids from Mouse Extrahepatic Bile Ducts. J. Vis. Exp. (146), e59544, doi:10.3791/59544 (2019).

Download .ris file

Copy

Share Video

.

Copy