Journal
/
Developmental Biology
/
Generación de organoides de conductos biliares extrahepáticos del ratón
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Generation of Organoids from Mouse Extrahepatic Bile Ducts
Please note that all translations are automatically generated. Click here for the English version.

Generación de organoides de conductos biliares extrahepáticos del ratón

español

Automatically Generated

9,426 Views

•

09:13 min

•

April 23, 2019

DOI:

10.3791/59544-v

DOI:
10.3791/59544-v

•

09:13 min
April 23, 2019

•

7 Views
, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Summary

Automatically generated

Este protocolo describe la producción de un sistema de 3 dimensiones organoide ratón vía biliar extrahepática. Estos organoides biliares se pueden mantener en cultivo a estudiar biología de cholangiocyte. Organoides biliares expresan marcadores de progenitoras y células biliares y están compuestas de células epiteliales polarizadas.

Explore More Videos

OrganoidsExtrahepatic Bile DuctsMurineCholangiopathiesPreclinical ModelsCholangiocyteCell-to-cell InteractionsBile Duct IsolationDissociationBasement MatrixCell Culture

Read Article

Cite this Article

Shiota, J., Zaki, N. H. M., Merchant, J. L., Samuelson, L. C., Razumilava, N. Generation of Organoids from Mouse Extrahepatic Bile Ducts. J. Vis. Exp. (146), e59544, doi:10.3791/59544 (2019).

Download .ris file

Copy

Share Video

.

Copy