Generation of Organoids from Mouse Extrahepatic Bile Ducts

Junya Shiota; Nureen  H. Mohamad Zaki; Juanita  L. Merchant; Linda  C. Samuelson; Nataliya Razumilava

doi:10.3791/59544

ジャーナル

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発生生物学

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Generation of Organoids from Mouse Extrahepatic Bile Ducts

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発生生物学

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JoVE Journal 発生生物学

Generation of Organoids from Mouse Extrahepatic Bile Ducts

9,426 Views

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09:13 min

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April 23, 2019

DOI:

10.3791/59544-v

DOI:

10.3791/59544-v

•

09:13 min

April 23, 2019

•

7 Views

Junya Shiota¹, Nureen H. Mohamad Zaki¹, Juanita L. Merchant^1,2, Linda C. Samuelson^1,2, Nataliya Razumilava¹

¹Departments of Internal Medicine,The University of Michigan, ²Molecular & Integrative Physiology,The University of Michigan

概要

This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.

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Generation of Organoids from Mouse Extrahepatic Bile Ducts

概要

Automatically generated

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