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Generation of Organoids from Mouse Extrahepatic Bile Ducts
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Organoids from Mouse Extrahepatic Bile Ducts

Generation of Organoids from Mouse Extrahepatic Bile Ducts

English

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9,426 Views

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09:13 min

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April 23, 2019

DOI:

10.3791/59544-v

DOI:
10.3791/59544-v

•

09:13 min
April 23, 2019

•

7 Views
, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Summary

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This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.

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OrganoidsExtrahepatic Bile DuctsMurineCholangiopathiesPreclinical ModelsCholangiocyteCell-to-cell InteractionsBile Duct IsolationDissociationBasement MatrixCell Culture

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Cite this Article

Shiota, J., Zaki, N. H. M., Merchant, J. L., Samuelson, L. C., Razumilava, N. Generation of Organoids from Mouse Extrahepatic Bile Ducts. J. Vis. Exp. (146), e59544, doi:10.3791/59544 (2019).

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