Journal
/
发育生物学
/
Generation of Organoids from Mouse Extrahepatic Bile Ducts
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Generation of Organoids from Mouse Extrahepatic Bile Ducts

Generation of Organoids from Mouse Extrahepatic Bile Ducts

9,426 Views

•

09:13 min

•

April 23, 2019

DOI:

10.3791/59544-v

DOI:
10.3791/59544-v

•

09:13 min
April 23, 2019

•

7 Views
, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Summary

Automatically generated

This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.

Explore More Videos

OrganoidsExtrahepatic Bile DuctsMurineCholangiopathiesPreclinical ModelsCholangiocyteCell-to-cell InteractionsBile Duct IsolationDissociationBasement MatrixCell Culture

Read Article

Cite this Article

Shiota, J., Zaki, N. H. M., Merchant, J. L., Samuelson, L. C., Razumilava, N. Generation of Organoids from Mouse Extrahepatic Bile Ducts. J. Vis. Exp. (146), e59544, doi:10.3791/59544 (2019).

Download .ris file

Copy

Share Video

.

Copy