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小鼠特拉切斯刷细胞的分离与定量评价

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Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

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小鼠特拉切斯刷细胞的分离与定量评价

DOI:

10.3791/59496-v

•

10:25 min

•

June 12, 2019

•

Saltanat Ualiyeva, Eri Yoshimoto, Nora A. Barrett, Lora G. Bankova

¹Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Chapters

00:04Title
01:24Preparation of Reagents
02:34Dissection of Mouse Trachea
04:47Tracheal Epithelial Digestion
08:31Results: Analysis of the Isolated Brush Cells from Mouse Tracheas
09:57Conclusion

Summary

Automatic Translation

刷细胞是罕见的胆碱能化学感觉上皮细胞发现在天真的小鼠气管。由于其数量有限,其功能在气道免疫和改造中的作用的体外评估具有挑战性。我们描述了一种通过流式细胞测定分离气管刷细胞的方法。

Tags

Tracheal Brush Cells Epithelial Isolation Papain Digestion Choline Acetyltransferase Fluorescent Reporter Mice Transcriptional Analysis RNA Sequencing Upper Airway Epithelium Dispase DNase I Tyrode’s Buffer FACS Buffer Euthanasia

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