Journal
/
Immunology and Infection
/
Isolatie en kwantitatieve evaluatie van de penseel cellen van muis Tracheas
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
Please note that all translations are automatically generated. Click here for the English version.

Isolatie en kwantitatieve evaluatie van de penseel cellen van muis Tracheas

Nederlands

Automatically Generated

7,034 Views

•

10:25 min

•

June 12, 2019

DOI:

10.3791/59496-v

DOI:
10.3791/59496-v

•

10:25 min
June 12, 2019

•

2 Views
, , ,
1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Summary

Automatically generated

Penseel cellen zijn zeldzame cholinerge chemosensorische epitheelcellen gevonden in de naïeve muis trachea. Vanwege hun beperkte aantallen is de ex vivo-evaluatie van hun functionele rol in luchtweg-immuniteit en-renovatie een uitdaging. We beschrijven een methode voor isolatie van tracheale penseel cellen door flow cytometrie.

Explore More Videos

Tracheal Brush CellsEpithelial IsolationPapain DigestionCholine AcetyltransferaseFluorescent Reporter MiceTranscriptional AnalysisRNA SequencingUpper Airway EpitheliumDispaseDNase ITyrode’s BufferFACS BufferEuthanasia

Read Article

Cite this Article

Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).

Download .ris file

Copy

Share Video

.

Copy