/
/
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
7,034 Views
•
10:25 min
•
June 12, 2019
DOI:
Summary
Automatically generated
Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.
Read Article
Cite this Article
Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).
Copy