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Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

6,992 Views

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10:25 min

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June 12, 2019

DOI:

10.3791/59496-v

DOI:

10.3791/59496-v

•

10:25 min

June 12, 2019

•

3 Views

Saltanat Ualiyeva, Eri Yoshimoto, Nora A. Barrett, Lora G. Bankova

¹Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Tags

Tracheal Brush Cells Epithelial Isolation Papain Digestion Choline Acetyltransferase Fluorescent Reporter Mice Transcriptional Analysis RNA Sequencing Upper Airway Epithelium Dispase DNase I Tyrode’s Buffer FACS Buffer Euthanasia

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