Journal
/
Immunology and Infection
/
Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker

Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker

DOI:
10.3791/59373-v

•

11:28 min

•

May 31, 2019

•

, , , , , , ,
1US Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services,National Wildlife Research Center, 2IDT Biologika GmbH,Am Pharmapark

Chapters

  • 00:04Title
  • 00:30Preparation of Solutions and Standards
  • 04:13Sample Preparation
  • 08:19LC-MS/MS Analysis
  • 09:04Results: Iophenoxic Acid Results in Mongoose Serum
  • 10:44Conclusion

Summary

Automatic Translation

Automatic Translation

We offered captive mongooses placebo oral rabies vaccine baits with ethyl or methyl iophenoxic acid as a biomarker and verified bait uptake using a novel liquid chromatography with tandem mass spectrometry (LC-MS/MS) method.

Tags

Iophenoxic AcidMongoose SerumBiological MarkerOral Rabies VaccinationProtein PrecipitationMethyl-IPAEthyl-IPAAnalytical MethodHPLCQuantificationParts Per Billion

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

Introducing Shear Stress in the Study of Bacterial Adhesion

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection

Intralymphatic Immunotherapy and Vaccination in Mice

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Read Article