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Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker

Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker

5,757 Views

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11:28 min

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May 31, 2019

DOI:

10.3791/59373-v

DOI:
10.3791/59373-v

•

11:28 min
May 31, 2019

•

1 Views
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1US Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services,National Wildlife Research Center, 2IDT Biologika GmbH,Am Pharmapark

Summary

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We offered captive mongooses placebo oral rabies vaccine baits with ethyl or methyl iophenoxic acid as a biomarker and verified bait uptake using a novel liquid chromatography with tandem mass spectrometry (LC-MS/MS) method.

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Iophenoxic AcidMongoose SerumBiological MarkerOral Rabies VaccinationProtein PrecipitationMethyl-IPAEthyl-IPAAnalytical MethodHPLCQuantificationParts Per Billion

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Cite this Article

Berentsen, A. R., Sugihara, R. T., Payne, C. G., Leinbach, I., Volker, S. F., Vos, A., Ortmann, S., Gilbert, A. T. Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker. J. Vis. Exp. (147), e59373, doi:10.3791/59373 (2019).

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