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Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker

Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (Herpestes Auropunctatus) Sera for Use as an Oral Rabies Vaccination Biological Marker

DOI:
10.3791/59373-v

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11:28 min

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May 31, 2019

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, , , , , , ,
1US Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services,National Wildlife Research Center, 2IDT Biologika GmbH,Am Pharmapark

Capitoli

  • 00:04Titolo
  • 00:30Preparation of Solutions and Standards
  • 04:13Sample Preparation
  • 08:19LC-MS/MS Analysis
  • 09:04Results: Iophenoxic Acid Results in Mongoose Serum
  • 10:44Conclusion

Summary

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We offered captive mongooses placebo oral rabies vaccine baits with ethyl or methyl iophenoxic acid as a biomarker and verified bait uptake using a novel liquid chromatography with tandem mass spectrometry (LC-MS/MS) method.

Tags

Iophenoxic AcidMongoose SerumBiological MarkerOral Rabies VaccinationProtein PrecipitationMethyl-IPAEthyl-IPAAnalytical MethodHPLCQuantificationParts Per Billion

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