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Isolamento, propagação e expressão de proteína príon durante a diferenciação Neuronal de células-tronco humanas polpa Dental
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Automatic Translation

Aqui nós apresentamos um protocolo para isolamento de células-tronco polpa Dental humana e propagação a fim de avaliar a expressão da proteína príon durante o processo de diferenciação neuronal.

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