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Isolatie, vermeerdering en Prion eiwituitdrukking tijdens neuronale differentiatie van stamcellen van menselijke tandmerg
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Automatic Translation

Hier presenteren we een protocol voor menselijke tandheelkundige Pulp stamcellen isolatie en vermeerdering teneinde het prion-eiwit expressie tijdens neuronale differentiatie.

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