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人牙髓干细胞神经分化过程中的分离、繁殖及普利翁蛋白表达
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
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人牙髓干细胞神经分化过程中的分离、繁殖及普利翁蛋白表达

DOI:
10.3791/59282-v

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11:50 min

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March 18, 2019

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, , , , , , , ,
1Laboratory of Experimental Medicine and Environmental Pathology,Sabina Universitas – Rieti University Hub, 2Department of Experimental Medicine,Sapienza University of Rome, 3Department of Science Dentistry and Maxillofacial,Sapienza University of Rome

Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种人类牙髓干细胞分离和繁殖的方案, 以评估神经元分化过程中的 prion 蛋白表达。

Tags

Dental Pulp Stem CellsIsolationPropagationPrion Protein ExpressionNeuronal Differentiation

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