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Journal
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Biochemistry
/
プロテオーム的分類の単一分子レベルでの均一性の定量化
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Biochemistry
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JoVE Journal
Biochemistry
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Please note that all translations are automatically generated.
Click here for the English version.
プロテオーム的分類の単一分子レベルでの均一性の定量化
DOI:
10.3791/59199-v
•
08:29 min
•
April 19, 2019
•
Simon Leclerc
2
,
Youri Arntz
,
Yuichi Taniguchi
3
1
Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research
,
RIKEN
,
2
Laboratoire de Biomatériaux et Bioimagerie
,
INSERM
,
3
PRESTO, Japan Science and Technology Agency
Chapters
00:04
Title
01:33
Cell Lysis and Fluorescent Labeling
03:26
Proteome Separation and Recovery
03:51
Microscope Coverslip Preparation
05:46
Observation with Single Molecule Fluorescence Microscopy
06:07
Results: Labeling Homogeneity at the Single Molecule Level
07:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、単一分子レベルの複雑な蛋白質のサンプルのそれぞれのタンパク質種のラベリングの均質性を評価するためのプロトコルを提案する.
Tags
Protein Quantification
Single-molecule Analysis
Fluorescent Labeling
Heterogeneity
Molecular Concentration Assays
Diagnostic Methods
Protein Species Analysis
Data Reproducibility
Lysing Buffer
Tween-20
DTT
CHAPS
Cy3 NHS Ester Dye
HEPES-NaOH
Biotin-2-amine
EDC
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