/

/

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

JoVE Journal
Biochemie

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Biochemie

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

5,989 Views

•

08:29 min

•

April 19, 2019

DOI:

10.3791/59199-v

DOI:

10.3791/59199-v

•

08:29 min

April 19, 2019

•

2 Views

Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^1,3

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie,INSERM, ³PRESTO, Japan Science and Technology Agency

Summary

Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.

Explore More Videos

Protein Quantification Single-molecule Analysis Fluorescent Labeling Heterogeneity Molecular Concentration Assays Diagnostic Methods Protein Species Analysis Data Reproducibility Lysing Buffer Tween-20 DTT CHAPS Cy3 NHS Ester Dye HEPES-NaOH Biotin-2-amine EDC

Read Article