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Effektiv differentiering af humane pluripotente stamceller i leverceller
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Effektiv differentiering af humane pluripotente stamceller i leverceller

DOI:
10.3791/58975-v

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07:37 min

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June 11, 2019

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, ,
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute,Stanford University School of Medicine, 2Department of Developmental Biology,Stanford University School of Medicine

Chapters

  • 00:04Title
  • 00:45Preparation of Differentiation Media
  • 02:04Seed hPSCs onto Plates at Defined Densities for Differentiation
  • 04:39Differentiation of hPSCs into Endodermal Cells and Liver Progenitors
  • 05:37Results: Differentiating Human Pluripotent Stem Cells into Liver Cells
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en monolayer, serum-fri metode til effektivt at generere hepatocyte-lignende celler fra humane pluripotente stamceller (hPSCs) i 18 dage. Dette indebærer seks trin som hpscs sekventielt differentiere til mellemliggende celle-typer såsom primitive streak, definitive endoderm, posterior foregut og lever opløbet forfædre før dannelse hepatocyte-lignende celler.

Tags

Keywords: Human Pluripotent Stem CellsLiver CellsDifferentiationRegenerative MedicineLiver Bud ProgenitorsHepatocyte-like CellsDevelopmental Signaling PathwaysHigh PurityIMDMPVACDM2CDM3CDM4CDM5Small MoleculesGrowth FactorsMatrixHPSCDissociation Agent

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