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Kwantificering van antilichaam-afhankelijke versterking van het Zika-Virus in primaire menselijke cellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
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Kwantificering van antilichaam-afhankelijke versterking van het Zika-Virus in primaire menselijke cellen

Nederlands

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8,608 Views

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07:53 min

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January 18, 2019

DOI:

10.3791/58691-v

DOI:
10.3791/58691-v

•

07:53 min
January 18, 2019

•

5 Views
, , , ,
1Department of Microbiology,Boston University School of Medicine, 2National Emerging Infectious Diseases Laborator,Boston University School of Medicine, 3Department of Entomology,Kansas State University

Summary

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Beschrijven we een methode om te evalueren van het effect van reeds bestaande immuniteit tegen dengue virus op de Zika-virus-infectie met behulp van menselijk serum, primaire menselijke cellen en kwantificering van de infectie door kwantitatieve real-time polymerasekettingreactie.

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Asad, S., Feitosa-Suntheimer, F., Gold, A., Londono-Renteria, B., Colpitts, T. M. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (143), e58691, doi:10.3791/58691 (2019).

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