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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
 

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Article DOI: 10.3791/58691-v • 07:53 min • January 18th, 2019
January 18th, 2019 •
1,2, 1,2, 1,2, 2, 1,2
1Department of Microbiology, Boston University School of Medicine, 2National Emerging Infectious Diseases Laborator, Boston University School of Medicine, 3Department of Entomology, Kansas State University

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résumé
Automatic Translation

We describe a method to evaluate the effect of pre-existing immunity against dengue virus on the Zika virus infection by using human serum, primary human cells, and infection quantification by quantitative real-time polymerase chain reaction.

Tags

Quantification Antibody-dependent Enhancement Zika Virus Primary Human Cells Pre-existing Immunity Dengue Virus Human Patient Serum Immune Cells Viral Infection Levels Data Interpretation Cell Culture Medium 96-well Plate Serial Dilutions Serum Samples Zika Virus Strains Stock Solution Multiplicity Of Infection
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