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Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

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07:53 min

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January 18, 2019

DOI:

10.3791/58691-v

DOI:
10.3791/58691-v

•

07:53 min
January 18, 2019

•

5 Views
, , , ,
1Department of Microbiology,Boston University School of Medicine, 2National Emerging Infectious Diseases Laborator,Boston University School of Medicine, 3Department of Entomology,Kansas State University

Summary

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We describe a method to evaluate the effect of pre-existing immunity against dengue virus on the Zika virus infection by using human serum, primary human cells, and infection quantification by quantitative real-time polymerase chain reaction.

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Antibody-dependent EnhancementZika VirusPrimary Human CellsDengue VirusPre-existing ImmunitySerum SamplesVirus InfectionCell CultureImmune ComplexesCell LysisQuantification

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Asad, S., Feitosa-Suntheimer, F., Gold, A., Londono-Renteria, B., Colpitts, T. M. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (143), e58691, doi:10.3791/58691 (2019).

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