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Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
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JoVE 杂志 免疫与感染
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

DOI:
10.3791/58691-v

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07:53 min

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January 18, 2019

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, , , ,
1Department of Microbiology,Boston University School of Medicine, 2National Emerging Infectious Diseases Laborator,Boston University School of Medicine, 3Department of Entomology,Kansas State University

Chapters

  • 00:04Title
  • 00:59Cell Seeding and Infection Setup
  • 02:46RNA Extraction and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) Analysis
  • 05:58Results: Dengue Virus (DENV) Immune Sera Enhances Zika Virus (ZIKV) Infection in Primary Human Macrophages
  • 06:50Conclusion

Summary

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We describe a method to evaluate the effect of pre-existing immunity against dengue virus on the Zika virus infection by using human serum, primary human cells, and infection quantification by quantitative real-time polymerase chain reaction.

Tags

Antibody-dependent EnhancementZika VirusPrimary Human CellsDengue VirusPre-existing ImmunitySerum SamplesVirus InfectionCell CultureImmune ComplexesCell LysisQuantification

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