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Journal
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Biochemistry
/
肾小球的分离及肾小球细胞表面蛋白的
活体标记
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Isolation of Glomeruli and
In Vivo
Labeling of Glomerular Cell Surface Proteins
Please note that all translations are automatically generated.
Click here for the English version.
肾小球的分离及肾小球细胞表面蛋白的
活体标记
DOI:
10.3791/58542-v
•
09:12 min
•
January 18, 2019
•
Eva Königshausen
,
Sebastian A. Potthoff
,
Raphael Haase
,
Catherine Meyer-Schwesinger
,
Ernest Kaufmann
,
L. Christian Rump
,
Johannes Stegbauer
,
Lorenz Sellin
,
Ivo Quack
,
Magdalena Woznowski
1
Department of Nephrology, Medical Faculty
,
Heinrich-Heine-University
,
2
Institute of Cellular and Integrative Physiology
,
University Medical Center Hamburg-Eppendorf
Chapters
00:04
Title
00:47
Surgical Procedure
03:00
In Vivo Biotinylation and Glomeruli Isolation
05:29
Glomeruli Washing and Quantification
06:25
Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins
08:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议的小鼠体内标记肾小球细胞表面蛋白与生物素
.
该协议包含有关如何灌注小鼠肾脏, 分离肾小球, 并执行内源性免疫沉淀的感兴趣的蛋白质的信息。
Tags
Glomeruli Isolation
In Vivo Labeling
Glomerular Cell Surface Proteins
Proteinuric Kidney Disease
Cell Surface Protein Trafficking
Organ Perfusion
Kidney Isolation
Aortic Perfusion
Renal Vein
PBS Perfusion
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