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Journal / Biochemistry / Isolation of Glomeruli and In Vivo Labeling of…
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Biochemistry

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JoVE Journal Biochemistry
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
 

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Article DOI: 10.3791/58542-v • 09:12 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 2, 1, 1, 1, 1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

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Summary
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Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

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Isolation Glomeruli In Vivo Labeling Glomerular Cell Surface Proteins Nephrology Field Cell Surface Protein Trafficking Proteinuric Kidney Disease Advantages Technique In-vivo Study Protein Analysis Organ Systems Isolation Techniques Mouse Anesthesia Disinfection Incision Abdominal Fascia Muscle Division Surgical Clamps Dissecting Microscope Visceral Organs Hepatic Phrenic Ligament Surgical Tweezers Suture Placement
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