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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

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09:12 min

January 18, 2019

DOI:

09:12 min
January 18, 2019

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Summary

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Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

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