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生物化学
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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
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生物化学
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JoVE 杂志 生物化学
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

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09:12 min

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January 18, 2019

DOI:

10.3791/58542-v

DOI:
10.3791/58542-v

•

09:12 min
January 18, 2019

•

9 Views
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1Department of Nephrology, Medical Faculty,Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology,University Medical Center Hamburg-Eppendorf

Summary

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Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

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Königshausen, E., Potthoff, S. A., Haase, R., Meyer-Schwesinger, C., Kaufmann, E., Rump, L. C., Stegbauer, J., Sellin, L., Quack, I., Woznowski, M. Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins. J. Vis. Exp. (143), e58542, doi:10.3791/58542 (2019).

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