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Kwantitatieve Polymerase Chain Reaction gebaseerde Analyses van lymfkliertest intestinale Microbiota na behandeling met mondelinge antibiotica
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
DOI:

08:33 min

November 17, 2018

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Chapters

  • 00:04Title
  • 00:39Administration of Antibiotics
  • 02:29Collection of Fecal Samples from Stool, Ileum Content and Ileum Wall
  • 05:16Quantification of Intestinal Microbiota by qPCR
  • 06:33Results: Quantification of Intestinal Microbiota by qPCR after Oral Antibiotic Treatment
  • 07:51Conclusion

Summary

Automatic Translation

Hier bieden wij gedetailleerde protocollen voor de orale toediening van antibiotica aan muizen, verzameling van fecale monsters, DNA-extractie en kwantificering van fecale bacteriën door qPCR.

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