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Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

9,955 Views

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11:37 min

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July 03, 2017

DOI:

10.3791/55536-v

DOI:
10.3791/55536-v

•

11:37 min
July 03, 2017

•

5 Views
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1Unité de Pathogénie Microbienne Moléculaire, INSERM U786,Institut Pasteur, 2Microbiologie et Maladies Infectieuses,Collège de France, 3Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163,Université Paris Descartes-Sorbonne Paris Cité

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ImmunofluorescenceStress GranuleBacterial ChallengeMammalian CellsCellular MicrobiologyPathogenStress Granule PathwayImage AnalysisS.flexneri M90TTryptic Soy AgarTryptic Soy BrothVirulence Gene ExpressionHeLa CellsInfection MediumGentamicin

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Vonaesch, P., Sansonetti, P. J., Schnupf, P. Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells. J. Vis. Exp. (125), e55536, doi:10.3791/55536 (2017).

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