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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

12,414 Views

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08:33 min

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November 17, 2018

DOI:

10.3791/58481-v

DOI:
10.3791/58481-v

•

08:33 min
November 17, 2018

•

14 Views
, ,
1The Peter Gorer Department of Immunobiology,King’s College London, 2The Francis Crick Institute

Summary

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Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.

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Cite this Article

Jimeno, R., Brailey, P. M., Barral, P. Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment. J. Vis. Exp. (141), e58481, doi:10.3791/58481 (2018).

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