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Antibiotic Dereplication Using the Antibiotic Resistance Platform
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Immunology and Infection
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JoVE Journal Immunology and Infection
Antibiotic Dereplication Using the Antibiotic Resistance Platform

Antibiotic Dereplication Using the Antibiotic Resistance Platform

English

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10,055 Views

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10:49 min

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October 17, 2019

DOI:

10.3791/60536-v

DOI:
10.3791/60536-v

•

10:49 min
October 17, 2019

•

25 Views
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1Department of Biochemistry and Biomedical Sciences, M.G. DeGroote Institute for Infectious Disease Research,McMaster University, 2Department of Molecular and Cellular Biology,University of Guelph

Summary

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We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.

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Cite this Article

Zubyk, H. L., Cox, G., Wright, G. D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019).

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