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口服抗生素治疗小鼠肠道微生物群的定量聚合酶链反应分析
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
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口服抗生素治疗小鼠肠道微生物群的定量聚合酶链反应分析

DOI:
10.3791/58481-v

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08:33 min

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November 17, 2018

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, ,
1The Peter Gorer Department of Immunobiology,King’s College London, 2The Francis Crick Institute

Chapters

  • 00:04Title
  • 00:39Administration of Antibiotics
  • 02:29Collection of Fecal Samples from Stool, Ileum Content and Ileum Wall
  • 05:16Quantification of Intestinal Microbiota by qPCR
  • 06:33Results: Quantification of Intestinal Microbiota by qPCR after Oral Antibiotic Treatment
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提供了详细的方案, 口服抗生素给小鼠, 收集粪便样本, dna 提取和定量的粪便细菌的 qpcr。

Tags

Quantitative PCRMicrobiotaOral Antibiotic TreatmentInflammatory Bowel DiseaseAntibiotic CocktailDrinking WaterStool SampleWeight LossMicrobial Dysbiosis

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