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单分子力谱中蛋白质共价固定化的研究

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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单分子力谱中蛋白质共价固定化的研究

DOI:

10.3791/58167-v

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11:13 min

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August 20, 2018

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Tanja D. Becke^2,3, Stefan Ness, Stefanie Sudhop³, Hermann E. Gaub, Markus Hilleringmann, Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Chapters

00:04Title
00:39Immobilization of Proteins via Functional Silane Coupling Agents
04:42Atomic Force Microscopy Based Single Molecule Force Spectroscopy
08:38Results: Single Force Spectroscopy
10:07Conclusion

Summary

Automatic Translation

本协议描述了 heterobifunctional 硅烷偶联剂在硅氧化物表面上的共价固定化, 其作用是以原子力显微镜为基础的单分子力光谱学为例。RrgA (pilus-1 尖黏附)与纤连蛋白的相互作用。

Tags

Covalent Immobilization Protein Single Molecule Force Spectroscopy Atomic Force Microscope Cantilever Probe Biomolecule Immobilization Silanization EDC NHS Phosphate-buffered Saline

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