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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
1Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, 3Center for Nano Science,Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen
Chapters
- 00:04Title
- 00:39Immobilization of Proteins via Functional Silane Coupling Agents
- 04:42Atomic Force Microscopy Based Single Molecule Force Spectroscopy
- 08:38Results: Single Force Spectroscopy
- 10:07Conclusion
This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.
