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Cultura e transfecção de células primárias de Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
Culture and Transfection of Zebrafish Primary Cells

Cultura e transfecção de células primárias de Zebrafish

português

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11,965 Views

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08:51 min

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August 17, 2018

DOI:

10.3791/57872-v

DOI:
10.3791/57872-v

•

08:51 min
August 17, 2018

•

13 Views
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1Division of Cellular and Molecular Neurobiology, Zoological Institute,Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics,Braunschweig University of Technology

Summary

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Apresentamos um protocolo eficiente e fácil de usar para a preparação de culturas de células primárias de zebrafish embriões para transfeccao e imagens de células vivas, bem como um protocolo para preparar as células primárias do cérebro adulto zebrafish.

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Cite this Article

Russo, G., Lehne, F., Pose Méndez, S. M., Dübel, S., Köster, R. W., Sassen, W. A. Culture and Transfection of Zebrafish Primary Cells. J. Vis. Exp. (138), e57872, doi:10.3791/57872 (2018).

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