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Culture and Transfection of Zebrafish Primary Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Culture and Transfection of Zebrafish Primary Cells

Culture and Transfection of Zebrafish Primary Cells

DOI:
10.3791/57872-v

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08:51 min

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August 17, 2018

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, , , , ,
1Division of Cellular and Molecular Neurobiology, Zoological Institute,Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics,Braunschweig University of Technology

Capitoli

  • 00:04Titolo
  • 00:39Preparation of Primary Cells from Zebrafish Embryos
  • 01:54Preparation of Reusable Poly-L-lysine-coated Glass Bottom Dishes
  • 03:26Preparation and Plating of Primary Cells
  • 06:02Transfection of Primary Cells with Plasmid DNA
  • 06:57Results: Live Cell Imaging of Zebrafish Primary Cells
  • 08:25Conclusion

Summary

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We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.

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Keywords: ZebrafishPrimary CellsCell CultureTransfectionIntercellular DynamicsCell LinesEmbryosChorion RemovalGlass-bottom DishesPoly-L-lysine Coating

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