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Cultura e transfezione di cellule primarie di Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
Culture and Transfection of Zebrafish Primary Cells
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Cultura e transfezione di cellule primarie di Zebrafish

italiano

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11,965 Views

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08:51 min

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August 17, 2018

DOI:

10.3791/57872-v

DOI:
10.3791/57872-v

•

08:51 min
August 17, 2018

•

13 Views
, , , , ,
1Division of Cellular and Molecular Neurobiology, Zoological Institute,Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics,Braunschweig University of Technology

Summary

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Vi presentiamo un protocollo efficiente e facile da usare per la preparazione di colture cellulari primarie di embrioni di zebrafish per la transfezione e live cell imaging nonché un protocollo per preparare cellule primarie dal cervello di zebrafish adulto.

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Cite this Article

Russo, G., Lehne, F., Pose Méndez, S. M., Dübel, S., Köster, R. W., Sassen, W. A. Culture and Transfection of Zebrafish Primary Cells. J. Vis. Exp. (138), e57872, doi:10.3791/57872 (2018).

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